Enzyme Development
Receive enzymes tailored to your specific application and performance requirements.
What's in for you?
- Enzymes with higher activity, specificity, and stability
- Enzymes tailored to your specific application and performance requirements
- Cost-reduction through efficient approach
- Maximized success rates through integrated rational enzyme engineering and biodiversity-driven discovery
- Flexible development in the most suitable expression host with application-relevant testing
- Complex biocatalysis challenges are solved with the help of an experienced partner and customized engineering strategies.
Design winners - Rational enzyme engineering
Enzyme engineering starts with gene synthesis, cloning, and expression of your lead enzyme in a microbial production strain.
Rather than limiting development to E. coli, select the most suitable expression strain from our established production strain portfolio to maximize the chances of success. In parallel, we develop and validate a robust enzyme assay tailored to your target properties, enabling reliable characterization throughout the engineering campaign.
Using a combination of bioinformatics, structural biology, and AI-driven predictions, we apply a rational enzyme engineering approach to optimize enzymes for your specific application.
Our comprehensive design toolbox includes large-scale sequence alignments, structure prediction, molecular docking, simulations, and AI-assisted hotspot identification to design focused enzyme libraries.
We generate targeted libraries of typically 100 to 1,000 variants and identify improved enzymes through multiparameter screening. For every variant, we document the underlying mutations together with the corresponding performance improvements.
The insights gained from each engineering cycle directly guide the next, combining updated bioinformatic analyses with the recombination of beneficial mutations through combinatorial mutagenesis. This iterative process continues until the desired performance is achieved.
Discovery & engineering - Unlock synergistic effects
Our enzyme engineering and enzyme discovery are based on the same bioinformatics toolbox. Therefore, a seamless transition between engineering and discovery and vice versa is possible. This offers new, exciting possibilities that make our enzyme engineering highly successful.
Before we start an enzyme engineering, we can scan nature’s diversity to find a better starting sequences that already largely exhibits the desired properties. During an enzyme engineering and the discovery of promising mutations, we can also analyze if any natural enzymes already carry those beneficial mutation.
In this way we can enrich enzyme engineering with exciting biodiversity from MetXtra™ and expand the accessible sequence space.
Flexible expression hosts and analytics
Enzyme engineering projects are not limited to E. coli as expression strain or simple assay readouts but can be performed in desired enzyme production strains and benefits from wide instrumental analytics and assay expertise.
Our semi-automated evaluation process ensures high accuracy while assessing application-relevant properties.
Challenges welcome!
We routinely apply and combine our broad expertise to deliver solutions on various targets including enzyme cascades, chemo-biocatalysis or uncommon enzyme classes.
Backed by a team with extensive experience in engineering enzymes and proteins for diverse industrial needs, we deliver tailored solutions to enhance your projects.
